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Design of experiments for development and optimization of a liquid chromatography coupled to tandem mass spectrometry bioanalytical assay
Author(s) -
Thorsteinsdóttir Unnur Arna,
Thorsteinsdóttir Margrét
Publication year - 2021
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4727
Subject(s) - chemistry , bioanalysis , chromatography , analyte , mass spectrometry , tandem mass spectrometry , tandem , liquid chromatography–mass spectrometry , resolution (logic) , analytical chemistry (journal) , computer science , materials science , artificial intelligence , composite material
Design of experiments (DoE) is a valuable tool for the optimization of quantitative bioanalytical methods utilizing liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Liquid chromatography mass spectrometry (LC–MS) is composed of several processes, including, liquid introduction and analyte ionization. The goal is to transfer analytes from atmospheric pressure to vacuum and maintain conditions that are compatible for both LC and MS. These processes involve many experimental factors which need to be simultaneously optimized to obtain maximum sensitivity and resolution at minimum retention time. In this tutorial, the basic concepts of DoE will be explained with focus on practical use of DoE. Three case studies optimized with DoE for liquid chromatography tandem mass spectrometry (LC–MS/MS) quantitative assays will then be presented.