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Screening the Q‐markers of TCMs from RA rat plasma using UHPLC‐QTOF/MS technique for the comprehensive evaluation of Wu‐Wei‐Wen‐Tong Capsule
Author(s) -
Jiang Hui,
Liu Jian,
Wang Yanling,
Chen Leijing,
Liu Hui,
Wang Zhen,
Wang Bin
Publication year - 2021
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4711
Subject(s) - chemistry , traditional medicine , icariin , flos , chromatography , baicalin , traditional chinese medicine , ginseng , biochemistry , high performance liquid chromatography , medicine , alternative medicine , pathology , rutin , antioxidant
The appropriate selection of quality marker (Q‐marker) for performing the comprehensive quality evaluation of traditional Chinese medicines (TCMs) has much more significance. Wu‐Wei‐Wen‐Tong Capsule (WWWTC), a TCMs prescription, is mainly utilized to treat rheumatoid arthritis (RA) in China. However, the comprehensive quality control for WWWTC has not been achieved because of lacking system analysis for the Q‐marker. In this study, a dual wavelength, 203 and 270 nm, was selected based on the feature of 15 Q‐markers, and a reliable UHPLC‐UV fingerprinting approach was established, achieving the comprehensive quality evaluation of WWWTC. First, we identified 91 prototypes in rat plasma after administering a set amount of WWWTC by using UHPLC‐QTOF/MS technique and selected them as the candidate Q‐markers. Next, based on the “five principles” of Q‐marker selection, 15 absorbed components among them including coumarin, cinnamic acid, cinnamaldehyde, cinnamic alcohol, and 2‐methoxycinnamaldehyde derived from Monarch medicine of Cmnamomi Mmulus ; epimedin C, icariin, baohuoside I, and anhydroicaritin derived from Monarch medicine Epimedii Folium ; germacrone, the sesquiterpene compound in Minister medicine Rhizoma Wenyujin Concisum ; pachymic acid, the tetracyclic triterpenoid acids in Assistant medicine Poria ; baicalin, baicalein, wogonin, and wogonoside in Guide medicine Scutellariae Radix , respectively, were seriously chosen as the Q‐markers, indicating preferable pharmacological effect on RA, characterization of transitivity and traceability as well as measurable components in WWWTC. The effective and meaningful strategy displayed a unique perspective for the exploration of Q‐markers in the quality evaluation and further ensured efficacy and safety of the TCMs.