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Novel approach to enhance sensitivity while retaining morphology in fragile tissue sections for mass spectrometry imaging
Author(s) -
GotoInoue Naoko,
Morisasa Mizuki,
Kimura Keisuke,
Mori Tsukasa
Publication year - 2020
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4670
Subject(s) - chemistry , mass spectrometry imaging , mass spectrometry , maldi imaging , desorption , matrix (chemical analysis) , matrix assisted laser desorption/ionization , analytical chemistry (journal) , chromatography , organic chemistry , adsorption
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) imaging is an effective tool for investigating the distribution of molecules. However, cryosections are made from non‐fixed tissues, causing difficulties in preparing sections from fragile, high‐water content tissues such as those from tadpoles. Here, we introduce a new method for preparing cryosections using an adhesive tape followed by transfer onto glass slides for MALDI‐MS imaging. Signals obtained from the transferred sections were higher than those from other sections, and the transferred sections had high optical quality. This novel approach could be an effective tool for MALDI‐MS imaging of aquatic animals.