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Elucidations on the structures of some putative flavonoids identified in postharvest withered grapes ( Vitis vinifera L.) by quadrupole time‐of‐flight mass spectrometry
Author(s) -
De Rosso Mirko,
Panighel Annarita,
Dalla Vedova Antonio,
Flamini Riccardo
Publication year - 2020
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4639
Subject(s) - chemistry , taxifolin , mass spectrometry , quadrupole time of flight , chromatography , tandem mass spectrometry , vitis vinifera , flavonoid , organic chemistry , botany , antioxidant , biology
Grape dehydration is an oenological process used for production of high‐quality reinforced and sweet wines. High‐resolution mass spectrometry (ultrahigh‐performance liquid chromatography/quadrupole time‐of‐flight [UHPLC/QTOF]) was used to deepen the characterization of some flavonoids previously proposed in Corvina and Raboso Piave withered grapes. By performing a targeted data analysis workflow and orthogonal identification approaches (tandem mass spectrometry [MS/MS]), in silico fragmentation, and calculation of putative retention time), elucidation on the structures of six compounds previously proposed was achieved (taxifolin‐pentoside, two tetrahydroxyflavanone‐hexoside derivatives, a tetrahydroxy‐dimethoxyflavanone‐hexoside derivative, a pentahydroxyflavone, and peonidin‐ O ‐pentoside); and the structures of four putative new grape flavonoids were characterized (dihydromyricetin‐ O ‐hexoside, taxifolin‐di‐ O ‐hexoside, isorhamnetin, and a pinoquercetin isomer). Findings enlarge the panorama of flavonoids in grape and of their possible biosynthetic pathways.