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Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective
Author(s) -
Rivera Emilio S.,
Djambazova Katerina V.,
Neumann Elizabeth K.,
Caprioli Richard M.,
Spraggins Jeffrey M.
Publication year - 2020
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4614
Subject(s) - chemistry , ion mobility spectrometry , mass spectrometry , biomolecule , mass spectrometry imaging , characterization (materials science) , workflow , molecular imaging , nanotechnology , computational biology , chromatography , computer science , database , biochemistry , materials science , microbiology and biotechnology , in vivo , biology
Imaging mass spectrometry (IMS) technologies are capable of mapping a wide array of biomolecules in diverse cellular and tissue environments. IMS has emerged as an essential tool for providing spatially targeted molecular information due to its high sensitivity, wide molecular coverage, and chemical specificity. One of the major challenges for mapping the complex cellular milieu is the presence of many isomers and isobars in these samples. This challenge is traditionally addressed using orthogonal liquid chromatography (LC)‐based analysis, though, common approaches such as chromatography and electrophoresis are not able to be performed at timescales that are compatible with most imaging applications. Ion mobility offers rapid, gas‐phase separations that are readily integrated with IMS workflows in order to provide additional data dimensionality that can improve signal‐to‐noise, dynamic range, and specificity. Here, we highlight recent examples of ion mobility coupled to IMS and highlight their importance to the field.