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In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging
Author(s) -
Fu Tingting,
Oetjen Janina,
Chapelle Manuel,
Verdu Alexandre,
Szesny Matthias,
Chaumot Arnaud,
DegliEsposti Davide,
Geffard Olivier,
Clément Yohann,
Salvador Arnaud,
Ayciriex Sophie
Publication year - 2020
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4531
Subject(s) - mass spectrometry , ion mobility spectrometry , isobaric process , mass spectrometry imaging , chemistry , ion , analytical chemistry (journal) , in situ , chromatography , ion mobility spectrometry–mass spectrometry , matrix assisted laser desorption/ionization , desorption , sample preparation in mass spectrometry , electrospray ionization , adsorption , physics , organic chemistry , thermodynamics
The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high‐resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.