In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging | Zendy

Fu Tingting | Zendy; Oetjen Janina | Zendy; Chapelle Manuel | Zendy; Verdu Alexandre | Zendy; Szesny Matthias | Zendy; Chaumot Arnaud | Zendy; DegliEsposti Davide | Zendy; Geffard Olivier | Zendy; Clément Yohann | Zendy; Salvador Arnaud | Zendy; Ayciriex Sophie | Zendy

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In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging

Author(s) -

Fu Tingting,

Oetjen Janina,

Chapelle Manuel,

Verdu Alexandre,

Szesny Matthias,

Chaumot Arnaud,

DegliEsposti Davide,

Geffard Olivier,

Clément Yohann,

Salvador Arnaud,

Ayciriex Sophie

Publication year - 2020

Publication title -

journal of mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 1076-5174

DOI - 10.1002/jms.4531

Subject(s) - chemistry , mass spectrometry , mass spectrometry imaging , isobaric process , ion mobility spectrometry , chromatography , maldi imaging , analytical chemistry (journal) , matrix assisted laser desorption/ionization , in situ , ion mobility spectrometry–mass spectrometry , ion , protein mass spectrometry , desorption , sample preparation in mass spectrometry , electrospray ionization , adsorption , organic chemistry , physics , thermodynamics

The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high‐resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.

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