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Immersion by rotation‐based application of the matrix for fast and reproducible sample preparations and robust results in mass spectrometry imaging
Author(s) -
Schäfermann Johanna,
Kliewer Georg,
Lösch Jan,
Bednarz Hanna,
Giampà Marco,
Niehaus Karsten
Publication year - 2020
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4488
Subject(s) - chemistry , mass spectrometry , mass spectrometry imaging , matrix (chemical analysis) , analyte , maldi imaging , ion suppression in liquid chromatography–mass spectrometry , nozzle , analytical chemistry (journal) , matrix assisted laser desorption/ionization , chromatography , desorption , tandem mass spectrometry , organic chemistry , adsorption , physics , thermodynamics
Automated matrix deposition for matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is crucial for producing reproducible analyte ion signals. Here we report an innovative method employing an automated immersion apparatus, which enables a robust matrix deposition within 5 minutes and with scalable throughput by using MAPS matrix and non‐polar solvents. MSI results received from mouse heart and rat brain tissues were qualitatively similar to those from nozzle sprayed samples with respect to peak number and quality of the ion images. Overall, the immersion‐method enables a fast and careful matrix deposition and has the future potential for implementation in clinical tissue diagnostics.