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Toward high pressure miniature protein mass spectrometer: Theory and initial results
Author(s) -
Hou Chenyue,
Xu Qian,
Zhang Fei,
Jiang Ting,
Xu Wei
Publication year - 2019
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4466
Subject(s) - mass spectrometry , chemistry , ion trap , ion , miniaturization , quadrupole ion trap , spectrometer , buffer gas , analytical chemistry (journal) , resolution (logic) , nanotechnology , chromatography , physics , optics , materials science , laser , organic chemistry , artificial intelligence , computer science
Current miniature mass spectrometers mainly focus on the analyses of organic and small biological molecules. In this study, we explored the possibility of developing high resolution miniature ion trap mass spectrometers for whole protein analysis. Theoretical derivation, GPU assisted ion trajectory simulation, and initial experiments on home‐developed “brick” mass spectrometer were carried out. Results show that ion‐neutral collisions have smaller damping effect on large protein ions, and a higher buffer gas pressure should be applied during ion trap operations for protein ions. As a result, higher pressure ion trap operation not only benefits instrument miniaturization, but also improves mass resolution of protein ions. Dynamic mass scan rate and generation of low charge state protein ions are also found to be helpful in terms of improving mass resolutions. Theory and conclusions found in this work are also applicable in the development of benchtop mass spectrometers.