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Complexes of cadmium ion with guanine bases detected by electrospray ionization mass spectrometry
Author(s) -
Moriwaki Hiroshi
Publication year - 2003
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.444
Subject(s) - chemistry , guanosine , guanine , electrospray ionization , cadmium , deoxyguanosine , mass spectrometry , chromatography , nucleotide , organic chemistry , biochemistry , adduct , gene
The complexations of cadmium ion with guanine bases were detected by electrospray ionization mass spectrometry (ESI‐MS). In order to explore the toxicity of cadmium, such as oxidative stress to DNA and carcinogenesis, it is very important to determine the interactions between the cadmium ion and nucleotide. The analysis of mixed cadmium ion–guanosine aqueous solution (molar ratio 1 : 9) using ESI‐MS (cone voltage 20 V) showed the presence of various cadmium complex ions, such as [ n (guanosine) + Cd] 2+ ( n = 3–8), [2guanine + Cd] 2+ , [guanosine + guanine + Cd] 2+ and [guanosine + 2guanine + Cd] 2+ . The observed [2guanine + Cd] 2+ , [guanosine + guanine + Cd] 2+ and [guanosine + guanine + Cd] 2+ ions are formed through the dissociation of the N–glycoside bond at the interface of ESI‐MS. For deoxyguanosine and ethylguanine, similar cadmium complexes were observed. However, the complexes between the cadmium ion and 8‐hydroxydeoxyguanosine were not detected. Furthermore, when a higher molar ratio (Cd : guanosine) or cone voltage were used, more of the monovalent ion peaks, such as [Cd(guanine − H) 2 + H] + and [Cd(guanosine − H) 2 + H] + , were observed and a decrease in the abundance of the divalent ions, such as [ n (guanosine)+Cd] 2+ , occurred. Copyright © 2003 John Wiley & Sons, Ltd.