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Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs
Author(s) -
Wang Bo,
Pang Maoda,
Zhao Xia,
Xie Kaizhou,
Zhang Peiyang,
Zhang Genxi,
Zhang Tao,
Liu Xuezhong,
Dai Guojun
Publication year - 2019
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4355
Subject(s) - thiamphenicol , florfenicol , chemistry , chromatography , extraction (chemistry) , chloramphenicol , electrospray ionization , electrospray , mass spectrometry , european union , antibiotics , biochemistry , business , economic policy
Accelerated solvent extraction was investigated as a novel alternative technology for the separation and quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine from poultry eggs, and the results were compared with the results of liquid–liquid extraction. Rapid quantification of the target compounds was carried out by ultra‐performance liquid chromatography‐electrospray ionization tandem triple quadrupole mass spectrometry. This optimized method was validated according to the requirements defined by the European Union and the United States Food and Drug Administration. Finally, the new approach was successfully applied to the quantitative determination of these analytes in 90 commercial poultry eggs from local supermarkets.