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Combining MALDI‐2 and transmission geometry laser optics to achieve high sensitivity for ultra‐high spatial resolution surface analysis
Author(s) -
Spivey Eric C.,
McMillen Josiah C.,
Ryan Daniel J.,
Spraggins Jeffrey M.,
Caprioli Richard M.
Publication year - 2019
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4335
Subject(s) - chemistry , mass spectrometry , maldi imaging , laser , orbitrap , analyte , ionization , resolution (logic) , optics , matrix assisted laser desorption/ionization , analytical chemistry (journal) , ion , desorption , physics , chromatography , organic chemistry , adsorption , artificial intelligence , computer science
A transmission geometry optical configuration allows for smaller laser spot size to facilitate high‐resolution matrix‐assisted laser/desorption ionization (MALDI) mass spectrometry. This increase in spatial resolution (ie, smaller laser spot size) is often associated with a decrease in analyte signal. MALDI‐2 is a post‐ionization technique, which irradiates ions and neutrals generated in the initial MALDI plume with a second orthogonal laser pulse, and has been shown to improve sensitivity. Herein, we have modified a commercial Orbitrap mass spectrometer to incorporate a transmission geometry MALDI source with MALDI‐2 capabilities to improve sensitivity at higher spatial resolutions.