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Imaging mass spectrometry of gold nanoparticles in a tissue section as an immunohistochemical staining mass probe
Author(s) -
Muko Daiki,
Ikenaga Takanori,
Kasai Masanori,
Rabor Janice B.,
Nishitani Atsushi,
Niidome Yasuro
Publication year - 2019
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4290
Subject(s) - chemistry , colloidal gold , autofluorescence , mass spectrometry , conjugated system , mass spectrometry imaging , fluorescence , primary and secondary antibodies , biophysics , analytical chemistry (journal) , nanoparticle , antibody , chromatography , nanotechnology , materials science , physics , organic chemistry , quantum mechanics , immunology , biology , polymer
For analysis of low abundance peptides in a tissue section, immunohistochemical staining through antibody‐antigen interaction is a usual technique. The antibody is conjugated with a probe moiety that aids in highly sensitive detection. Gold nanoparticles, which show excellent chemical stability and variation of surface modifications, are expected to act as a sensitive mass probe to desorb gold ions (Au + , Au 2 + , Au 3 + ) that are distinguishable from fragment ions from organic molecules. Here, green fluorescent proteins (GFP) in a tissue section of a transgenic zebrafish were detected by the gold mass probe conjugated with antibodies. Due to the efficient ionization and desorption of gold ions, imaging mass spectrometry of Au 2 + ions indicated the distribution of gold nanoparticles stained in a tissue section, and the mass signal distribution was consistent with the area where the GFP‐expressing cells were distributed. Conventional immunofluorescence techniques showed intense autofluorescence that come from intrinsic fluorophores in the tissue section. In contrast, the gold nanoparticles acted as an immunostaining mass probe that displayed significantly lower background signals.