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Fragmentation of the main triterpene acids of apple by LC‐APCI‐MS n
Author(s) -
Sut Stefania,
Poloniato Gabriele,
Malagoli Mario,
Dall'Acqua Stefano
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4264
Subject(s) - chemistry , triterpene , atmospheric pressure chemical ionization , chromatography , mass spectrometry , terpene , betulinic acid , fragmentation (computing) , ursolic acid , liquid chromatography–mass spectrometry , high performance liquid chromatography , chemical ionization , oleanolic acid , tandem mass spectrometry , organic chemistry , ionization , ion , medicine , alternative medicine , pathology , biology , computer science , genetics , operating system
In this paper, we investigated the fragmentation of the main triterpene acids of apple using an liquid chromatography atmospheric pressure chemical ionization mass spectrometry (LC‐APCI‐MS n ) approach and high‐resolution mass spectrometry (HR‐MS) (Q‐TOF). Triterpenes were isolated using semipreparative high‐performance liquid chromatography, and chemical structures were elucidated by HR‐MS and nuclear magnetic resonance spectroscopy. Finally, compounds were used to study MS n behavior in ion trap. Isolated triterpenes present similar structures, bearing carboxyl group linked to C‐17 and different substitutions. We observed significant changes in MS 2 spectra, which were useful for further compound identification. The observed fragments allowed the discrimination of different derivatives, namely, pomaceic, annurcoic, euscaphic, pomolic, corosolic, maslinic, betulinic, oleanolic, and ursolic acids. The proposed method allows a rapid identification of triterpene acids, and it could be useful for the analysis of these compounds in apple fruits and in other natural sources.