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Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds
Author(s) -
Brkljačić Lidija,
Jerić Ivanka
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4201
Subject(s) - chemistry , derivatization , reagent , hydrazone , fragmentation (computing) , benzaldehyde , hydrazine (antidepressant) , dissociation (chemistry) , chromatography , mass spectrometry , collision induced dissociation , hexanal , tandem mass spectrometry , organic chemistry , combinatorial chemistry , catalysis , computer science , operating system
Chemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by liquid chromatography‐tandem mass spectrometry analysis. The main requirements regarding the good derivatization reagent are fast, selective, and quantitative reaction with carbonyl compounds and efficient fragmentation upon collision‐induced dissociation. We have prepared glutamic acid‐related hydrazine reagent and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The collision‐induced dissociation spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure‐specific fragment ions were selected for the development of sensitive multiple reaction monitoring method.