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Quantification of DNA methylation and hydroxymethylation in Alzheimer's disease mouse model using LC‐MS/MS
Author(s) -
Huang Qionglin,
Xu Simin,
Mo Mingming,
Yang Qingjin,
Li Jian,
Zhong Yu,
Zhang Junjie,
Zhang Lijian,
Ye Xiaoxiao,
Liu Zhou,
Cai Chun
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4194
Subject(s) - chemistry , dna methylation , epigenetics , 5 methylcytosine , genomic dna , methylation , dna , alzheimer's disease , disease , neuroscience , medicine , biochemistry , gene , psychology , gene expression
Ambiguous alteration patterns of 5‐methylcytosine (5mC) and 5‐hydroxymethylcytosine (5hmC) involved in Alzheimer's disease (AD) obstructed the mechanism investigation of this neurological disorder from epigenetic view. Here, we applied a fully quantitative and validated LC‐MS/MS method to determine genomic 5mC and 5hmC in the brain cortex of 3 month‐aged (12, 15, and 18 month) AD model mouse and found significant increases of 5mC and 5hmC levels in different months of AD mouse when compared with age‐matched wild‐type control and exhibited rising trend from 12‐month to 18‐month AD mouse, thereby supporting genomic DNA methylation and hydroxymethylation were positively correlated with developing AD.