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Silver‐109‐based laser desorption/ionization mass spectrometry method for detection and quantification of amino acids
Author(s) -
Arendowski Adrian,
Nizioł Joanna,
Ruman Tomasz
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4068
Subject(s) - chemistry , mass spectrometry , chromatography , detection limit , monoisotopic mass , matrix assisted laser desorption/ionization , mass spectrometry imaging , surface enhanced laser desorption/ionization , sample preparation in mass spectrometry , amino acid , protein mass spectrometry , capillary electrophoresis–mass spectrometry , desorption , resolution (logic) , analytical chemistry (journal) , tandem mass spectrometry , electrospray ionization , adsorption , organic chemistry , biochemistry , artificial intelligence , computer science
A new methodology applicable for both high‐resolution laser desorption/ionization mass spectrometry and mass spectrometry imaging of amino acids is presented. The matrix‐assisted laser desorption ionization‐type target containing monoisotopic cationic 109 Ag nanoparticles ( 109 AgNPs) was used for rapid mass spectrometry measurements of 11 amino acids of different chemical properties. Amino acids were directly tested in 100,000‐fold concentration change conditions ranging from 100 μg/mL to 1 ng/mL which equates to 50 ng to 500 fg of amino acid per measurement spot. Limit of detection values obtained suggest that presented method/target system is among the fastest and most sensitive ones in laser mass spectrometry. Mass spectrometry imaging of spots of human blood plasma spiked with amino acids showed their surface distribution allowing optimization of quantitative measurements.