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High‐resolution multiple reaction monitoring method for quantification of steroidal hormones in plasma
Author(s) -
Peti Ana Paula Ferranti,
Locachevic Gisele Aparecida,
Prado Morgana Kelly Borges,
Moraes Luiz Alberto Beraldo,
Faccioli Lúcia Helena
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3998
Subject(s) - chemistry , context (archaeology) , hormone , resolution (logic) , computational biology , high resolution , chromatography , biochemistry , biology , computer science , paleontology , remote sensing , artificial intelligence , geology
Steroidal hormones profiles can be used to distinguish different biological conditions by the amount of entities present especially in the context of disease. Knowing the levels of such molecules can be crucial to determining a biological response and/or unveil important phenomena. The emergence of high‐resolution methods has amplified the potential of MS and it is currently possible to identify numerous steroid species. In addition, sensitive and very specific modes of acquisition, such as high‐resolution multiple reaction monitoring (MRM), have contributed to this end. In this perspective special feature article, Lúcia Helena Faccioli and colleagues propose a high‐resolution MRM method to quantify steroidal hormones and their metabolites. The results indicate that the method can be applied for quantification of steroids in several biological samples, such as human plasma. Dr. Faccioli is Professor of immunology in the Dept. of Clinical Analyses, Toxicology and Bromatology at the University of Sao Paulo (Brazil). Her main research interests are centered on the study of inflammatory response and role of lipid mediators in several disease including tuberculosis. She also investigates natural products with anti‐inflammatory properties.