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Quaternary ammonium isobaric tag for a relative and absolute quantification of peptides
Author(s) -
Setner Bartosz,
Stefanowicz Piotr,
Szewczuk Zbigniew
Publication year - 2018
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3989
Subject(s) - chemistry , ammonium , isobaric process , chromatography , isobaric labeling , bovine serum albumin , absolute (philosophy) , protein quaternary structure , mass spectrometry , biochemistry , organic chemistry , electrospray ionization , thermodynamics , protein mass spectrometry , philosophy , physics , epistemology , protein subunit , gene
In the ongoing quest to develop improved quantitative approaches for complex mixtures of proteins, this month Setner and colleagues describe a strategy that combines an isobaric tag for relative and absolute quantification with the incorporation of a quaternary ammonium group to increase ionization efficiency. This strategy ‐ they call QAS‐iTRAQ 2‐plex ‐ can be adopted for both relative and absolute quantification of peptides. The authors provide an abundance of data in support of their approach, and describe its application to a series of model peptides and a tryptic digest of bovine serum albumin.