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Myofiber metabolic type determination by mass spectrometry imaging
Author(s) -
Centeno Delphine,
Vénien Annie,
PujosGuillot Estelle,
Astruc Thierry,
Chambon Christophe,
Théron Laëtitia
Publication year - 2017
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3957
Subject(s) - chemistry , glycolysis , mass spectrometry , fiber type , myocyte , matrix assisted laser desorption/ionization , mass spectrometry imaging , oxidative phosphorylation , biochemistry , biophysics , metabolism , fiber , chromatography , microbiology and biotechnology , desorption , biology , organic chemistry , adsorption
Matrix assisted laser desorption/ionization (MALDI) mass spectrometry imaging is a powerful tool that opens new research opportunities in the field of biology. In this work, predictive model was developed to discriminate metabolic myofiber types using the MALDI spectral data. Rat skeletal muscles are constituted of type I and type IIA fiber, which have an oxidative metabolism for glycogen degradation, and type IIX and type IIB fiber which have a glycolytic metabolism, present in different proportions according to the muscle function and physiological state. So far, myofiber type is determined by histological methods that are time consuming. Thanks to the predictive model, we were able to predict not only the metabolic fiber type but also their location, on the same muscle section that was used for MALDI imaging. Copyright © 2017 John Wiley & Sons, Ltd.