A one‐step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms | Zendy

Li Bin | Zendy; Comi Troy J. | Zendy; Si Tong | Zendy; Dunham Sage J. B. | Zendy; Sweedler Jonathan V. | Zendy

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A one‐step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms

Author(s) -

Li Bin,

Comi Troy J.,

Si Tong,

Dunham Sage J. B.,

Sweedler Jonathan V.

Publication year - 2016

Publication title -

journal of mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 1076-5174

DOI - 10.1002/jms.3827

Subject(s) - chemistry , mass spectrometry imaging , biofilm , mass spectrometry , maldi imaging , matrix (chemical analysis) , matrix assisted laser desorption/ionization , chromatography , bacteria , organic chemistry , adsorption , desorption , biology , genetics

Matrix‐assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one‐step, spray‐based application of a 2,5‐dihydroxybenzoic acid solution for direct matrix‐assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home‐built automatic sprayer, region‐specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm‐promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar‐based biofilms. Copyright © 2016 John Wiley & Sons, Ltd.

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