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Influence of mobile phase, source parameters and source type on electrospray ionization efficiency in negative ion mode
Author(s) -
Kruve Anneli
Publication year - 2016
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3790
Subject(s) - chemistry , electrospray ionization , ion source , ionization , mass spectrometry , extractive electrospray ionization , thermal ionization , chemical ionization , analytical chemistry (journal) , direct electron ionization liquid chromatography–mass spectrometry interface , ambient ionization , ion , electrospray , chromatography , electron ionization , sample preparation in mass spectrometry , organic chemistry
Electrospray ionization (ESI) efficiency is known to be affected by the properties of the analytes, source design and source parameters. In this study, the ionization efficiency of 17 acidic compounds at various conditions in ESI negative ion mode was evaluated. Namely, the influence of organic solvent content in the mobile phase, ionization source parameters, ionization source geometry and functionality (conventional ESI, ESI with thermal focussing and with additional internal nebulizer gas) was studied. It was observed that the ionization efficiency in thermal focussing ESI is only marginally affected by the organic solvent composition, while for conventional ESI and ESI with internal nebulizer gas, the ionization efficiency increases significantly with increasing organic modifier content. For all ionization sources and mobile phase compositions, the ionization efficiency values between different setups showed good correlation. Copyright © 2016 John Wiley & Sons, Ltd.