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Simultaneous determination of metabolites of trimethylbenzenes, dimethylbenzylmercapturicacid and dimethylhippuric acid, in human urine by solid‐phase extraction followed by liquid chromatography tandem mass spectrometry
Author(s) -
Moriwaki Hiroshi,
Watanabe Ayumi,
Arakawa Ryuichi,
Tsujimoto Yuji,
Shimizu Mitsuru,
Noda Tsutomu,
Warashina Munehiro,
Tanaka Masanobu
Publication year - 2002
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.378
Subject(s) - chemistry , chromatography , solid phase extraction , analyte , mass spectrometry , tandem mass spectrometry , electrospray ionization , selected reaction monitoring , liquid chromatography–mass spectrometry , electrospray , urine , detection limit , extraction (chemistry) , sample preparation , biochemistry
We describe a novel method for the determination of three kinds of dimethylbenzylmercapturic acids (DMM) and six kinds of dimethylhippuric acids (DMH), found in urine as metabolites of trimethylbenzenes, based on liquid chromatography/electrospray ionization tandem mass spectrometry. A solid‐phase extraction procedure was used for the extractions of DMM and DMH from a urine sample, and the separation was performed on a reversed–phase C 30 column. The analytes were ionized by electrospray in the positive‐ion mode. Operating in the multiple reaction monitoring mode, the linearity of the relative mass spectrometric responses to the internal standard versus analyte concentrations were established in the range 0.1–100 ng ml −1 . The extraction procedure was rapid and the relative standard deviations were below 5%. The detection limits of DMM and DMH in the urine by the proposed method were in the ranges 0.26–0.41 and 0.42–2.0 ng l −1 , respectively. Furthermore, DMM and DMH were detected in a urine sample from an individual who did not suffer from occupational exposure to trimethylbenzenes, by using this method. Copyright © 2002 John Wiley & Sons, Ltd.