Premium
Laser ablation sample transfer for localized LC‐MS/MS proteomic analysis of tissue
Author(s) -
Donnarumma F.,
Murray K. K.
Publication year - 2016
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3660
Subject(s) - laser capture microdissection , chemistry , tissue sample , mass spectrometry , laser ablation , sample preparation , ablation , proteomics , laser , chromatography , analytical chemistry (journal) , optics , biomedical engineering , biochemistry , medicine , gene expression , physics , engineering , gene , aerospace engineering
Imaging mass spectrometry (IMS) has shown great potential as a tool to increase proteomic information obtained from tissue sections in a spatially resolved manner. One of the bottlenecks of the technology is rapid protein identification. Extraction of spatially localized regions of interest from tissue samples followed by protein analysis by LC‐MS/MS is a strategy that has shown potential in this regard. Further, analyzing tissue extracts has the advantage of providing identification of a large number of proteins. In this Special Feature article, Kermit Murray and colleagues demonstrate the potential of IR laser ablation sample transfer (IR LAST) for extraction of biological material from tissue sections mounted on a microscope slide by irradiation in transmission geometry for capture in a solvent located below the area of interest. They provide a comparison with UV laser capture microdissection and demonstrate that the captured material can be further processed for high throughput LC‐MS/MS proteomic analysis. Kermit Murray is professor of Chemistry at Louisiana State University (Baton Rouge, LA). His research is primarily aimed at MS instrument development.