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Visualization of cancer‐related chemical components in mouse pancreas tissue by tapping‐mode scanning probe electrospray ionization mass spectrometry
Author(s) -
Otsuka Yoichi,
Satoh Shuya,
Naito Junpei,
Kyogaku Masafumi,
Hashimoto Hiroyuki
Publication year - 2015
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3634
Subject(s) - chemistry , mass spectrometry imaging , mass spectrometry , electrospray ionization , analytical chemistry (journal) , electrospray , ionization , visualization , chemical ionization , chromatography , ion , organic chemistry , artificial intelligence , computer science
Mass spectrometry imaging is an informative approach for the comprehensive analysis of multiple components inside biological specimens. We used novel tapping‐mode scanning probe electrospray ionization mass spectrometry method to visualize cancer‐related chemical components in the mouse pancreas tissue section at a sampling pitch of 100 µm. Positive ion mode measurements from m / z 100 to 1500 resulted in the visualization of multiple components that are tentatively assigned as polyamines, lipids and proteins. Their signal intensities inside the cancerous and the non‐cancerous regions were found to be significantly different by the two‐sample t ‐test. Copyright © 2015 John Wiley & Sons, Ltd.