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Application of gas chromatography–tandem mass spectrometry (GC/MS/MS) for the analysis of deuterium enrichment of water
Author(s) -
Walker Dillon K.,
Thaden John J.,
Deutz Nicolaas E. P.
Publication year - 2015
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3593
Subject(s) - chemistry , deuterium , mass spectrometry , analytical chemistry (journal) , chromatography , gas chromatography , electron ionization , isotope dilution , tandem mass spectrometry , gas chromatography–mass spectrometry , ion , ionization , organic chemistry , physics , quantum mechanics
Incorporation of deuterium from deuterium oxide ( 2 H 2 O) into biological components is a commonly used approach in metabolic studies. Determining the dilution of deuterium in the body water (BW) pool can be used to estimate body composition. We describe three sensitive GC/MS/MS methods to measure water enrichment in BW. Samples were reacted with NaOH and U‐ 13 C 3 ‐acetone in an autosampler vial to promote deuterium exchange with U‐ 13 C 3 ‐acetone hydrogens. Headspace injections were made of U‐ 13 C 3 ‐acetone‐saturated air onto a 30‐m DB‐1MS column in electron impact‐mode. Subjects ingested 30 ml 2 H 2 O, and plasma samples were collected. BW was determined by standard equation. Dual‐energy X‐ray absorptiometry scans were performed to calculate body mass, body volume and bone mineral content. A four‐compartmental model was used to estimate body composition (fat and fat free mass). Full‐scan experiments generated an m / z 45 peak and to a lesser extent an m / z 61 peak. Product fragment ions further monitored included 45 and 46 using selected ion monitoring (Method1), the 61 > 45 and 62 > 46 transition using multiple reaction monitoring (MRM; Method2) and the neutral loss, 62 > 45, transition (Method3). MRM methods were optimized for collision energy (CE) and collision‐induced dissociation (CID) argon gas pressure with 6 eV CE and 1.5 mTorr CID gas being optimal. Method2 was used for final determination of 2 H 2 O enrichment of subjects because of lower natural background. We have developed a sensitive method to determine 2 H 2 O enrichment in BW to enable measurement of FM and FFM. Copyright © 2015 John Wiley & Sons, Ltd.