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Direct chemical profiling of olive ( Olea europaea ) fruit epicuticular waxes by direct electrospray‐ultrahigh resolution mass spectrometry
Author(s) -
Vichi Stefania,
CortésFrancisco Nuria,
Romero Agustí,
Caixach Josep
Publication year - 2015
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3562
Subject(s) - chemistry , olea , epicuticular wax , mass spectrometry , electrospray ionization , wax , orbitrap , electrospray , chromatography , analytical chemistry (journal) , botany , organic chemistry , biology
In the present paper, an electrospray ionization (ESI)‐Orbitrap method is proposed for the direct chemical profiling of epicuticular wax (EW) from Olea europaea fruit. It constitutes a rapid and efficient tool suitable for a wide‐ranging screening of a large number of samples. In a few minutes, the method provides a comprehensive characterization of total EW extracts, based on the molecular formula of their components. Accurate mass measurements are obtained by ultrahigh resolution mass spectrometry, and compositional restrictions are set on the basis of the information available from previous studies of olive EW. By alternating positive and negative ESI modes within the same analysis, complementary results are obtained and a wide range of chemical species is covered. This provides a detailed compositional overview that otherwise would only be available by applying multiple analytical techniques. Copyright © 2015 John Wiley & Sons, Ltd.