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Combining UV photodissociation with electron transfer for peptide structure analysis
Author(s) -
Shaffer Christopher J.,
Marek Ales,
Pepin Robert,
Slovakova Kristina,
Turecek Frantisek
Publication year - 2015
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3551
Subject(s) - chemistry , photodissociation , electron transfer dissociation , electron transfer , electron capture dissociation , dissociation (chemistry) , diazirine , photochemistry , ion , covalent bond , fragmentation (computing) , peptide , chromophore , tandem mass spectrometry , mass spectrometry , organic chemistry , chromatography , biochemistry , computer science , operating system
The combination of near‐UV photodissociation with electron transfer and collisional activation provides a new tool for structure investigation of isolated peptide ions and reactive intermediates. Two new types of pulse experiments are reported. In the first one called UV/Vis photodissociation–electron transfer dissociation (UVPD‐ETD), diazirine‐labeled peptide ions are shown to undergo photodissociation in the gas phase to form new covalent bonds, guided by the ion conformation, and the products are analyzed by electron transfer dissociation. In the second experiment, called ETD‐UVPD wherein synthetic labels are not necessary, electron transfer forms new cation–peptide radical chromophores that absorb at 355 nm and undergo specific backbone photodissociation reactions. The new method is applied to distinguish isomeric ions produced by ETD of arginine containing peptides. Copyright © 2015 John Wiley & Sons, Ltd.