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One drop chemical derivatization – DESI‐MS analysis for metabolite structure identification
Author(s) -
Lubin Arnaud,
Cabooter Deirdre,
Augustijns Patrick,
Cuyckens Filip
Publication year - 2015
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3489
Subject(s) - derivatization , chemistry , bioanalysis , metabolite , analyte , metabolite profiling , chromatography , drug discovery , mass spectrometry , active metabolite , metabolomics , biochemistry
Metabolite identification is a crucial part of the discovery and development process of new pharmaceutical drugs. Structural elucidation of metabolites can be valuable for the optimization of lead candidates in drug discovery and provide critical information to assess the toxicology or pharmacology profile of compounds in drug development. In this special feature article, Filip Cuyckens and co‐workers present an easy to set up and time saving method for metabolite identification where the analyte in solution is simply dried on a glass plate with printed Teflon spots on which a single drop of derivatization mixture is added. Once the spot has dried, the derivatized compound is analyzed by DESI‐MS and the observed mass shifts induced by the derivatization allow the analyst to deduce the presence or absence of the investigated functional group(s). Dr. Filip Cuyckens' main research interests are in novel analytical approaches for metabolite profiling and identification and bioanalysis.