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DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta
Author(s) -
AlBalaa Dania,
Rajchl Aleš,
Grégrová Adéla,
Ševčík Rudolf,
Čížková Helena
Publication year - 2014
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3465
Subject(s) - chemistry , detection limit , chromatography , mass spectrometry , dart ion source , extraction (chemistry) , gas chromatography , saponification , flame ionization detector , solvent , time of flight mass spectrometry , analytical chemistry (journal) , ionization , ion , electron ionization , organic chemistry
To ensure that egg‐containing products, such as dried eggs and egg pasta, conform to the technological and legislative requirements for egg content, methods are needed to determine the amount of cholesterol in such products. The conventional approach, direct saponification and hexane extraction followed by cholesterol determination by gas chromatography coupled to a flame ionization detector, is very time consuming. Therefore, we developed a rapid method on the basis of direct analysis in real time coupled to time‐of‐flight mass spectrometry. Samples were prepared simply by solvent extraction followed by extract filtration. The optimization of certain parameters, including the solvent used and direct analysis in real time ionization gas temperature, had a pronounced effect on the intensities of the produced ions, in particular, the molecular and dehydrated ions of cholesterol and its deuterated analog, cholesterol 2,2,3,4,4,6‐ d 6 , which was used as an internal standard. For the developed method, limits of detection and quantification were 0.03 and 0.05 mg g −1 , respectively. The results of the real samples were compared with those obtained using the conventional approach [limit of detection = 0.002 mg g −1 and limit of quantification = 0.05 mg g −1 ], and it was found that, although the results obtained using the conventional approach were more accurate, our developed method is much simpler and faster, where the time was dramatically reduced by 87% for executing a screening analysis. Copyright © 2014 John Wiley & Sons, Ltd.