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Determination of clindamycin in animal plasma by high‐performance liquid chromatography combined with electrospray ionization mass spectrometry
Author(s) -
Cherlet Marc,
Croubels Siska,
De Backer Patrick
Publication year - 2002
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.344
Subject(s) - chemistry , chromatography , detection limit , electrospray ionization , ammonium acetate , mass spectrometry , electrospray , high performance liquid chromatography
A method for the quantification of clindamycin in animal plasma using high‐performance liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI‐MS/MS) is presented. Lincomycin is used as the internal standard. The sample preparation includes a simple deproteinization step with trichloroacetic acid. Chromatographic separation is achieved on an RP‐18 Hypersil column using gradient elution with 0.01 M ammonium acetate and acetonitrile as mobile phase. Good linearity was observed in the range 0–10 µg ml −1 . The limit of quantification of the method is 50 ng ml −1 and the limit of detection is 1.3 ng ml −1 . The method was shown out to be of use for pharmacokinetic studies of clindamycin formulations in dogs. Copyright © 2002 John Wiley & Sons, Ltd.