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Determination of ivermectin B 1a in animal plasma by liquid chromatography combined with electrospray ionization mass spectrometry
Author(s) -
Croubels S.,
De Baere S.,
Cherlet M.,
De Backer P.
Publication year - 2002
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.343
Subject(s) - chemistry , chromatography , detection limit , electrospray ionization , mass spectrometry , acetic acid , calibration curve , electrospray , selected reaction monitoring , analytical chemistry (journal) , tandem mass spectrometry , biochemistry
A novel, sensitive and specific method for the quantitative determination of ivermectin B 1a in animal plasma using liquid chromatography combined with positive electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) is presented. Abamectin was used as the internal standard. Extraction of the samples was performed with a deproteinization step using acetonitrile. Chromatographic separation was achieved on a Nucleosil ODS 5 µm column, using gradient elution with 0.2% (v/v) acetic acid in water and 0.2% (v/v) acetic acid in acetonitrile. The method was validated according to the requirements defined by the European Community. Calibration curves using plasma fortified between 1 and 100 ng ml −1 showed a good linear correlation ( r ≥ 0.9989, goodness‐of‐fit coefficient ≤8.1%). The trueness at 2 and 25 ng ml −1 ( n = 6) was +4.2 and −17.1%, respectively. The trueness and between‐run precision for the analysis of quality control samples at 25 ng ml −1 was −4.0 and 11.0%, respectively ( n = 16). The limit of quantification of the method was 1.0 ng ml −1 , for which the trueness and precision also fell within acceptable limits. Using a signal‐to‐noise ratio of 3 : 1, the limit of detection was calculated to be 0.2 ng ml −1 . The specificity was demonstrated with respect to ivermectin B 1b . The method was successfully used for the quantitative determination of ivermectin B 1a in plasma samples from treated bovines, demonstrating the usefulness of the developed method for application in the field of pharmacokinetics. Copyright © 2002 John Wiley & Sons, Ltd.