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N‐hydroxysuccinimidyl p‐methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food
Author(s) -
Mazzotti Fabio,
Di Donna Leonardo,
Napoli Anna,
Aiello Donatella,
Siciliano Carlo,
Athanassopoulos Constantinos M.,
Sindona Giovanni
Publication year - 2014
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3417
Subject(s) - chemistry , reagent , derivatization , chromatography , isotope dilution , mass spectrometry , tandem mass spectrometry , chemical ionization , ionization , organic chemistry , ion
We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope‐coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N‐hydroxysuccinimidyl ester of d 0 /d 4 ‐4‐methoxybenzoic acid (d 0 /d 4 ‐4‐MBA‐OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix‐assisted laser desorption/ionization–mass spectrometry (MS) and MS/MS. Multiple‐reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters. Copyright © 2014 John Wiley & Sons, Ltd.