N‐hydroxysuccinimidyl p‐methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food | Zendy

Mazzotti Fabio | Zendy; Di Donna Leonardo | Zendy; Napoli Anna | Zendy; Aiello Donatella | Zendy; Siciliano Carlo | Zendy; Athanassopoulos Constantinos M. | Zendy; Sindona Giovanni | Zendy

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N‐hydroxysuccinimidyl p‐methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food

Author(s) -

Mazzotti Fabio,

Di Donna Leonardo,

Napoli Anna,

Aiello Donatella,

Siciliano Carlo,

Athanassopoulos Constantinos M.,

Sindona Giovanni

Publication year - 2014

Publication title -

journal of mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 1076-5174

DOI - 10.1002/jms.3417

Subject(s) - chemistry , reagent , derivatization , chromatography , isotope dilution , mass spectrometry , tandem mass spectrometry , chemical ionization , ionization , organic chemistry , ion

We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope‐coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N‐hydroxysuccinimidyl ester of d 0 /d 4 ‐4‐methoxybenzoic acid (d 0 /d 4 ‐4‐MBA‐OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix‐assisted laser desorption/ionization–mass spectrometry (MS) and MS/MS. Multiple‐reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters. Copyright © 2014 John Wiley & Sons, Ltd.

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