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MALDI‐MS analysis and imaging of small molecule metabolites with 1,5‐diaminonaphthalene (DAN)
Author(s) -
Korte Andrew R.,
Lee Young Jin
Publication year - 2014
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3400
Subject(s) - chemistry , mass spectrometry , metabolite , chromatography , matrix (chemical analysis) , matrix assisted laser desorption/ionization , phospholipid , desorption , organic chemistry , biochemistry , adsorption , membrane
1,5‐diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix‐assisted laser desorption ionization‐mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metabolite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9‐aminoacridine (9‐AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9‐AA. Finally, DAN and 9‐AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.