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Application of 13 C isotope labeling using liquid chromatography mass spectrometry (LC‐MS) to determining phosphate‐containing metabolic incorporation
Author(s) -
Bhowmik Salil Kumar,
Putluri Vasanta,
Kommagani Ramakrishna,
Konde Sai Aparna,
Lydon John P.,
Sreekumar Arun,
Putluri Nagireddy
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3292
Subject(s) - chemistry , mass spectrometry , chromatography , glycolysis , electrospray ionization , isotope , phosphate , isotopes of carbon , flux (metallurgy) , metabolism , metabolic flux analysis , isotopic labeling , radiochemistry , biochemistry , organic chemistry , physics , quantum mechanics , total organic carbon
Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C‐labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2‐Deoxyglucose. Copyright © 2013 John Wiley & Sons, Ltd.