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Alternative two‐step matrix application method for imaging mass spectrometry to avoid tissue shrinkage and improve ionization efficiency
Author(s) -
Shimma Shuichi,
Takashima Yuki,
Hashimoto Jun,
Yonemori Kan,
Tamura Kenji,
Hamada Akinobu
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3288
Subject(s) - chemistry , mass spectrometry imaging , mass spectrometry , shrinkage , matrix (chemical analysis) , chromatography , one step , analytical chemistry (journal) , chemical engineering , machine learning , computer science , engineering
Mass spectrometry (MS) was used to measure the concentrations of drug and biological compounds in plasma and tissues. Matrix‐assisted laser desorption/ionization (MALDI) imaging MS (IMS) has recently been applied to the analysis of localized drugs on biological tissue surfaces. In MALDI‐IMS, matrix application process is crucial for successful results. However, it is difficult to obtain homogeneous matrix crystals on the tissue surface due to endogenous salts and tissue surface heterogeneity. Consequently, the non‐uniform crystals degrade the quality of the spectrum and likely cause surface imaging artifacts. Furthermore, the direct application of matrix solution can cause tissue shrinkage due to the organic solvents. Here, we report an alternative two‐step matrix application protocol which combines the vacuum deposition of matrix crystals and the spraying of matrix solution to produce a homogeneous matrix layer on the tissue surface. Our proposed technique can also prevent cracking or shrinking of the tissue samples and improve the ionization efficiency of the distributed exogenous material. Copyright © 2013 John Wiley & Sons, Ltd.