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A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry
Author(s) -
Manier M. L.,
Spraggins J. M.,
Reyzer M. L.,
Norris J. L.,
Caprioli R. M.
Publication year - 2014
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3273
Subject(s) - derivatization , chemistry , mass spectrometry imaging , mass spectrometry , endogeny , chromatography , amine gas treating , metabolite , metabolomics , amino acid , metabolite profiling , computational biology , biochemistry , organic chemistry , biology
Imaging Mass Spectrometry is increasingly being applied to the study of small endogenous compounds, including metabolites, neurotransmitters, lipids and other compounds as well. However, due to the high degree of structural homology and the lack of true “blank” samples, generation of images of unequivocal molecular identity is challenging. In this special feature perspective article, Richard Caprioli and colleagues at Vanderbilt University Medical Center discuss these challenges and describe an analytical strategy that combines a number of advanced instrumental methods to identify and confirm the accurate spatial localization of select amino acids and amine‐containing metabolites. By combining derivatization, MS n , and accurate mass, followed by confirmation via HPLC‐MS, they are able to demonstrate the localization of several endogenous metabolites in biological tissue specimens.