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Extraction protocol and mass spectrometry method for quantification of doxorubicin released locally from prodrugs in tumor tissue
Author(s) -
Ibsen Stuart,
Su Yongxuan,
Norton John,
Zahavy Eran,
Hayashi Tomoko,
Adams Stephen,
Wrasidlo Wolf,
Esener Sadik
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3221
Subject(s) - doxorubicin , chemistry , prodrug , biodistribution , mass spectrometry , chromatography , extraction (chemistry) , chemotherapy , biochemistry , in vitro , medicine , surgery
The localized conversion of inactive doxorubicin prodrug chemotherapeutics to pharmacalogically active forms is difficult to quantify in mouse tumor models because it occurs only in small regions of tissue. The tumor tissue extraction protocol and LC–MS/MS analysis method described here were optimized to obtain a detection limit of 7.8 pg for the activated doxorubicin and 0.36 ng for the doxorubicin prodrug. This method can be useful for determining the biodistribution and activation efficiency for many different doxorubicin prodrugs. It can also be used for quantification of doxorubicin from tumor models that have poor vascularization resulting in low tissue accumulation. Copyright © 2013 John Wiley & Sons, Ltd.