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Modified MALDI MS fatty acid profiling for bacterial identification
Author(s) -
Voorhees Kent J.,
Jensen Kirk R.,
McAlpin Casey R.,
Rees Jon C.,
Cody Robert,
Ubukata Masaaki,
Cox Christopher R.
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3215
Subject(s) - chemistry , derivatization , chromatography , matrix assisted laser desorption/ionization , mass spectrometry , ambient ionization , ionization , mass spectrum , chemical ionization , analytical chemistry (journal) , ion , desorption , organic chemistry , adsorption
Bacterial fatty acid profiling is a well‐established technique for bacterial identification. Ten bacteria were analyzed using both positive‐ and negative‐ion modes with a modified matrix‐assisted laser desorption ionization mass spectrometry (MALDI MS) approach using CaO as a matrix replacement (metal oxide laser ionization MS (MOLI MS)). The results show that reproducible lipid cleavage similar to thermal in situ tetramethyl ammonium hydroxide saponification/derivatization had occurred. Principal component analysis showed that replicates from each organism grouped in a unique space. Cross validation (CV) of spectra from both ionization modes resulted in greater than 94% validation of the data. When CV results were compared for the two ionization modes, negative‐ion data produced a superior outcome. MOLI MS provides clinicians a rapid, reproducible and cost‐effective bacterial diagnostic tool. Copyright © 2013 John Wiley & Sons, Ltd.
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