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Protein analysis by desorption electrospray ionization mass spectrometry and related methods
Author(s) -
Douglass K. A.,
Venter A. R.
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3188
Subject(s) - desorption electrospray ionization , chemistry , desorption , mass spectrometry , ionization , extractive electrospray ionization , analyte , electrospray ionization , analytical chemistry (journal) , electrospray , field desorption , ion , molecule , ambient ionization , chromatography , chemical ionization , chemical physics , sample preparation in mass spectrometry , organic chemistry , adsorption
As a tool for analyzing solid samples Desorption Electrospray Ionization MS (DESI‐MS) offers some very attractive features: samples require minimal treatment and can remain at atmosphere during the measurement, which have fueled the growth of the technique. In recent years there have been numerous applications for using DESI to analyze a variety of compounds and surfaces which show DESI analysis of larger protein molecules can still present a challenge, particularly when high sensitivity is required. The droplet pickup model of ion formation has been proposed for protein desorption/ionization whereby the charged primary spray interacts with the sample surface to dissolve and desorb analyte molecules followed by desolvation and charge transfer. In the Special Feature, Professor Andre Venter and Kevin Douglass from Western Michigan University deconstruct the DESI process with a particular focus on large proteins and examine the efficiency of the desorption and ionization processes separately, in order to better understand of how each process contributes to the overall detection efficiency of large protein ions.