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An effective and rapid determination by MALDI/TOF/TOF of methionine sulphoxide content of ApoA‐I in type 2 diabetic patients
Author(s) -
Seraglia Roberta,
Sartore Giovanni,
Marin Raffaella,
Burlina Silvia,
Manzato Enzo,
Ragazzi Eugenio,
Traldi Pietro,
Lapolla Annunziata
Publication year - 2013
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3113
Subject(s) - chemistry , methionine , apolipoprotein b , cholesterol , lipoprotein , oxidative stress , high density lipoprotein , mass spectrometry , low density lipoprotein , matrix assisted laser desorption/ionization , biochemistry , chromatography , organic chemistry , amino acid , adsorption , desorption
Increased oxidation of low density lipoprotein (LDL) is characteristic of atherosclerosis. In this frame, high density lipoproteins (HDL) play an important role, being able to remove lipid peroxides (LPOs) and cholesterol from oxidized LDL, so exhibiting a protective role against atherosclerosis. A wide range of reactive compounds lead to the oxidation of methionine (Met) residues with the formation of methionine sulphoxide (MetO) in apolipoprotein A‐I (ApoA‐I). Consequently, the determination of MetO level can give both an evaluation of oxidative stress and the reduced capability of ApoA‐I in LPOs and cholesterol transport. For these reasons, the development of analytical methods able to determine the MetO level is surely of interest, and we report here the results obtained by MALDI mass spectrometry. Copyright © 2013 John Wiley & Sons, Ltd.