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PSAQ™ standards for accurate MS–based quantification of proteins: from the concept to biomedical applications
Author(s) -
Picard Guillaume,
Lebert Dorothée,
Louwagie Mathilde,
Adrait Annie,
Huillet Céline,
Vandenesch François,
Bruley Christophe,
Garin Jérôme,
Jaquinod Michel,
Brun Virginie
Publication year - 2012
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3111
Subject(s) - chemistry , proteomics , quantitative proteomics , absolute (philosophy) , nanotechnology , analytical chemistry (journal) , chromatography , biochemistry , philosophy , materials science , epistemology , gene
With the development of the Protein Standard Absolute Quantification (PSAQ method), the use of isotopically labeled analogue standards and MS analysis was transferred from the field of small molecules to the field of proteomics analysis. In this article, Dr Virginie Brun and colleagues focus on the use of PSAQ standards for absolute quantification of biomedically relevant proteins. They start by describing the research project which first led to develop the PSAQ technology and then present the methods used to produce, control the quality of and use these labeled proteins for absolute MS‐based quantification. Finally, the main current applications of the PSAQ method are illustrated.