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Separation of isomeric disaccharides by traveling wave ion mobility mass spectrometry using CO 2 as drift gas
Author(s) -
Fasciotti Maíra,
Sanvido Gustavo B.,
Santos Vanessa G.,
Lalli Priscila M.,
McCullagh Michael,
Sá Gilberto F.,
Daroda Romeu J.,
Peter Martin G.,
Eberlin Marcos N.
Publication year - 2012
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3089
Subject(s) - chemistry , disaccharide , ion mobility spectrometry , maltose , cellobiose , mass spectrometry , analytical chemistry (journal) , ion , sucrose , chromatography , lactose , resolution (logic) , polarizability , molecule , stereochemistry , organic chemistry , hydrolysis , artificial intelligence , computer science , cellulase
The use of CO 2 as a massive and polarizable drift gas is shown to greatly improve peak‐to‐peak resolution ( R p‐p ), as compared with N 2 , for the separation of disaccharides in a Synapt G2 traveling wave ion mobility cell. Near or baseline R p‐p was achieved for three pairs of sodiated molecules of disaccharide isomers, that is, cellobiose and sucrose ( R p‐p = 0.76), maltose and sucrose ( R p‐p = 1.04), and maltose and lactose ( R p‐p = 0.74). Ion mobility mass spectrometry using CO 2 as the drift gas offers therefore an attractive alternative for fast and efficient separation of isomeric disaccharides. Copyright © 2012 John Wiley & Sons, Ltd.