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Analysis of chloroquine and metabolites directly from whole‐body animal tissue sections by liquid extraction surface analysis (LESA) and tandem mass spectrometry
Author(s) -
Parson Whitney B.,
Koeniger Stormy L.,
Johnson Robert W.,
Erickson Jamie,
Tian Yu,
Stedman Christopher,
Schwartz Annette,
Tarcsa Edit,
Cole Roderic,
Van Berkel Gary J.
Publication year - 2012
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3068
Subject(s) - chemistry , chromatography , pharmacokinetics , tandem mass spectrometry , mass spectrometry , extraction (chemistry) , sample preparation , liquid chromatography–mass spectrometry , penetration (warfare) , pharmacology , medicine , operations research , engineering
The rapid and direct analysis of the amount and spatial distribution of exogenous chloroquine (CHQ) and CHQ metabolites from tissue sections by liquid extraction surface sampling analysis coupled with tandem mass spectrometry (LESA‐MS/MS) was demonstrated. LESA‐MS/MS results compared well with previously published CHQ quantification data collected by organ excision, extraction and fluorescent detection. The ability to directly sample and analyze spatially resolved exogenous molecules from tissue sections with minimal sample preparation and analytical method development has the potential to facilitate the assessment of target tissue penetration of pharmaceutical compounds, to establish pharmacokinetic/pharmacodynamic relationships, and to complement established pharmacokinetic methods used in the drug discovery process during tissue distribution assessment. Copyright © 2012 John Wiley & Sons, Ltd.