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Lipid A structure of Pseudoalteromonas haloplanktis TAC 125: use of electrospray ionization tandem mass spectrometry for the determination of fatty acid distribution
Author(s) -
Corsaro Maria Michela,
Piaz Fabrizio Dal,
Lanzetta Rosa,
Parrilli Michelangelo
Publication year - 2002
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.304
Subject(s) - chemistry , glucosamine , residue (chemistry) , electrospray ionization , tandem mass spectrometry , disaccharide , fatty acid , electrospray , chromatography , mass spectrometry , lipid a , amino sugar , biochemistry , bacteria , biology , genetics
The use of electrospray Ionization (ESI) tandem mass spectrometry (MS/MS) for the structural determination of the lipid A components of the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 is reported. The lipid A contains the classical bisphosphorylated β‐(1′ → 6)‐linked D ‐glucosamine disaccharide with 3‐hydroxydodecanoyl residues (12 : 0 (3‐OH)) linked both as esters and amides to 2′, 3′ (distal glucosamine) and 2, 3 positions (proximal glucosamine) of the sugar backbone. The hydroxyl of 12 : 0 (3‐OH) fatty acid linked at the 3′ position is esterified by a dodecanoyl residue (12 : 0). In addition to the pentaacyl component, a minor tetraacyl lipid A, lacking the acyl residue at position 3, was also found in the lipid A fraction. The advantage of this MS technique for the investigation of the intra‐ring fragmentation, which is useful for the determination of fatty acyl residue distribution on each glucosamine unit, is emphasized. Copyright © 2002 John Wiley & Sons, Ltd.