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Quantitative analysis of memantine in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry
Author(s) -
Leis Hans Jörg,
Fauler Günter,
Windischhofer Werner
Publication year - 2002
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.303
Subject(s) - chemistry , chromatography , mass spectrometry , chemical ionization , memantine , detection limit , selected ion monitoring , gas chromatography–mass spectrometry , selected reaction monitoring , amantadine , gas chromatography , ionization , analytical chemistry (journal) , ion , tandem mass spectrometry , nmda receptor , biochemistry , receptor , organic chemistry , neuroscience , biology
A sensitive and specific method for the determination of memantine in human plasma is presented. Memantine was extracted from plasma and derivatized to the pentafluorobenzoyl derivative in a one‐step procedure avoiding any sample concentration steps. Adamantine was used as an internal standard. The compounds were measured by gas chromatography/negative ion chemical ionization mass spectrometry without any further processing. Using this detection mode, the fragment ions at m / z 353 and 325 were obtained at high relative abundance. Calibration graphs were linear over the range 0.117–30 ng ml −1 . At the limit of quantification (LOQ), the inter‐assay precision was 2.00% and the intra‐assay variability was 3.22%. The accuracy at the LOQ showed deviations of −1.42% (intra‐assay) and −2.47% (inter‐assay). The method is rugged, rapid and robust and was applied to the batch determination of memantine during pharmacokinetic profiling of the drug. Copyright © 2002 John Wiley & Sons, Ltd.