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Seed oil triglyceride profiling of thirty‐two hybrid grape varieties
Author(s) -
De Marchi Fabiola,
Seraglia Roberta,
Molin Laura,
Traldi Pietro,
De Rosso Mirko,
Panighel Annarita,
Dalla Vedova Antonio,
Gardiman Massimo,
Giust Mirella,
Flamini Riccardo
Publication year - 2012
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3010
Subject(s) - chemistry , triglyceride , chromatography , electrospray ionization , mass spectrometry , food science , solvent , organic chemistry , biochemistry , cholesterol
Triglyceride profile of seed oil samples from 32 hybrid grape varieties not studied before was investigated. A new method for the analysis of triacylglycerols (TAGs) has been developed based on the direct infusion in the electrospray ionization (ESI) source and employing tetrahydrofuran/methanol/water (85:10:5 v|v|v) as solvent; the formation of [M + Na] + ions in high yield has been observed. TAGs were identified by ESI‐tandem mass spectrometry analysis, and the matrix‐assisted‐laser‐desorption‐ionization and time‐of‐flight profile of samples was determined. Six were the principal TAGs identified in seed oil: trilinolein (LLL) was the most abundant (43%), followed by dilinoleoyl‐oleoylglycerol (LOL, 23%), and dilinoleoyl‐palmitoylglycerol (LPL, 15%). Compounds present in lower concentration were LSL and LOO (11%), LOP (6%), and LSP (2%). Compared with seed oils produced from V. Vinifera grapes, some significant differences in the relative abundances of TAGs were found, in particular hybrid grape seed oils showed higher LOL and lower LPL content, respectively. Among the samples studied, a particularly high content of LLL (rich in unsaturated fatty acids) was found in seed oils from two red varieties. Copyright © 2012 John Wiley & Sons, Ltd.