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Secondary processes in atmospheric pressure chemical ionization–ion trap mass spectrometry: a case study of orotic acid
Author(s) -
Fryčák Petr,
Jirkovský Jaromír,
Ranc Václav,
Bednář Petr,
Havlíček Vladimír,
Lemr Karel
Publication year - 2012
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.3006
Subject(s) - chemistry , chemical ionization , dissociation (chemistry) , ion source , atmospheric pressure chemical ionization , ionization , protonation , mass spectrometry , deprotonation , ion , collision induced dissociation , ion trap , molecule , organic chemistry , tandem mass spectrometry , chromatography
Atmospheric pressure chemical ionization is known for producing unusual artifacts of the ionization process in some cases. In this work, processes occuring in atmospheric pressure chemical ionization/MS of orotic acid that afforded ions accompanying protonated and deprotonated orotic acid molecules in the spectra were studied. Two processes ran in parallel in the ion source: decarboxylation of neutral orotic acid and collision‐induced dissociation of its protonated or deprotonated form. A procedure discerning pre‐ionization decomposition and post‐ionization dissociation by manipulating ion source parameters was proposed. Experiments with isotopically labeled solvents confirmed ion–molecule reactions of the product of collision‐induced dissociation of protonated orotic acid with solvent molecules in the ion source and even under vacuum in the ion trap. Copyright © 2012 John Wiley & Sons, Ltd.