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Identification of adulteration in milk by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Cozzolino R.,
Passalacqua S.,
Salemi S.,
Malvagna P.,
Spina E.,
Garozzo D.
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.206
Subject(s) - chemistry , chromatography , mass spectrometry , raw milk , matrix assisted laser desorption/ionization , surface enhanced laser desorption/ionization , matrix (chemical analysis) , bovine milk , dairy industry , food science , desorption , protein mass spectrometry , electrospray ionization , organic chemistry , adsorption
The development is described of a rapid, simply and accurate analytical method aimed at evaluating both the presence of cow milk in either raw ewe and water buffalo milk samples employed in industrial processes and the addition of powdered milk to samples of fresh raw milk, using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS). The presence of adulteration is defined by evaluating the protein patterns coming from the most abundant whey proteins, α‐lactalbumin and β‐lactoglobulin, used as molecular markers. As no pretreatment of the milk samples is required and owing to the speed and ease of use of MALDI‐MS the proposed analytical protocol can be used as a routine strategy for the identification of possible adulteration of the raw fresh milk samples that the dairy industry receives from producers every day. Copyright © 2001 John Wiley & Sons, Ltd.