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Evaluation of relative LC/MS response of metabolites to parent drug in LC/nanospray ionization mass spectrometry: potential implications in MIST assessment
Author(s) -
Schadt Simone,
Chen LinZhi,
Bischoff Daniel
Publication year - 2011
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.2018
Subject(s) - chemistry , chromatography , metabolite , mass spectrometry , tandem mass spectrometry , quantitative analysis (chemistry) , active metabolite , biochemistry
There is an increasing demand for quantitative data on metabolite exposure triggered by regulatory guidances. This contribution describes the accuracy of nanoelectrospray ionization mass spectrometry response of drug compounds and their metabolites from biological matrices compared with radiometric quantification. This is a comprehensive investigation of a set of real‐life pharmaceutical compounds in relevant matrices such as urine, bile, feces and plasma. The data suggest that nanoelectrospray mass spectrometry can be used for semi‐quantitation of metabolites in the absence of reference standards. Therefore, this approach is suitable to screen out non‐relevant metabolites early in development as long as an adequate analytical error margin is applied thus balancing risks and resources. Copyright © 2011 John Wiley & Sons, Ltd.