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Surface‐assisted laser desorption and ionization mass spectrometry using low‐cost matrix‐free substrates
Author(s) -
Huo Haibin,
Shen Mengyan,
Ebstein Steven M.,
Guthermann Howard
Publication year - 2011
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1961
Subject(s) - chemistry , mass spectrometry , soft laser desorption , desorption , matrix (chemical analysis) , ionization , matrix assisted laser desorption/ionization , laser , matrix assisted laser desorption electrospray ionization , analytical chemistry (journal) , chromatography , ion , organic chemistry , adsorption , optics , physics
Surface‐assisted laser desorption/ionization (SALDI) substrates have been fabricated using nanospiked polyurethane (PU) substrates that are replicated by a low‐cost soft nanolithography method from silicon nanospike structures formed with femtosecond laser irradiations. The strongest mass spectrometry (MS) signal of Angiotensin II was obtained on 45‐nm Au‐coated nanospiked PU substrates. The effective ionization appears to be due to surface plasmon excitation. Such low‐cost and identical SALDI substrates can be used for MS analysis of various molecules with high reproducibility. Copyright © 2011 John Wiley & Sons, Ltd.